Simulated Microgravity Induces Cultivar-Specific Changes Affecting Salmonella enterica Ingression Independent of Stomatal Physiology

Abstract

Advances in NASAs astrobiology program have demonstrated the feasibility of cultivating plants in space and in analog extraterrestrial habitats. In addition to abiotic stressors, plants grown in terrestrial and space-like environments are challenged by both phytopathogens and opportunistic human pathogens, with implications for plant productivity and human health. The persistence of human-associated pathogens in spacecraft and space stations raises significant concerns regarding food safety. The molecular, biochemical, and signaling mechanisms governing stomatal development and function under microgravity remain poorly understood. We employed an experimental system incorporating human pathogen Salmonella enterica and lettuce microgreens exposed to simulated microgravity through two-dimensional clinorotation to investigate plant innate immunity and stomatal development and function. We further evaluated four lettuce cultivars to determine whether genetic variation impacts these factors under simulated microgravity conditions. Our findings indicate that simulated microgravity significantly influences stomatal development and function, as evidenced by an increase in stomatal density and variable changes to stomatal aperture. Notably, cultivar-dependent variation in stomatal traits and responses to Salmonella enterica was observed under microgravity conditions. Although increased stomatal density was hypothesized to enhance pathogen ingression, internalization was more strongly predicted by cultivar selection and simulated microgravity; simulated microgravity increased ingression, with red pigmented cultivars having less pathogen than green cultivars. These results suggest that targeted selection of cultivars with favorable physiological traits may improve food safety and the viability of crop production systems in space environments. They also suggest that development and function of stomata may change in spaceflight conditions.