Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer's Disease Cerebrocortical Organoids

Balistreri, A.; Turner, N.; Compher, J.; Almaraz, M.; Prabhavalkar, A.; Chittal, S.; Labra, S. R.; Ezekiel, K.; Baal, C.; Cedeno Kwong, C.; Ghatak, S.; Schaefer, J.-H.; Vanderpool, K.; Spencer, K.; Yates, J. R.; Nolan, J. P.; Henderson, S.; Lipton, S. A.; Kelly, J. W.

doi:10.64898/2026.05.13.724352

bioRxiv · 10.64898/2026.05.13.724352

Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer's Disease Cerebrocortical Organoids

Balistreri, A.·Turner, N.·Compher, J.·Almaraz, M.·Prabhavalkar, A.·Chittal, S.·Labra, S. R.·Ezekiel, K.·Baal, C.·Cedeno Kwong, C.·Ghatak, S.·Schaefer, J.-H.·Vanderpool, K.·Spencer, K.·Yates, J. R.·Nolan, J. P.·Henderson, S.·Lipton, S. A.·Kelly, J. W.

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Abstract

Alzheimers disease (AD) is a neurodegenerative disorder affecting millions of patients globally. Despite significant efforts from researchers in recent decades, there are still many unanswered questions about AD pathogenesis. AD patient brains manifest changes in extracellular vesicles (EVs) secreted from diseased neurons, and the effect of this phenomenon remains poorly understood. EVs contain a variety of biomolecules and play a critical role in cell-to-cell communication in all eukaryotic organisms. Here, we report a thorough characterization of small EVs purified from cultures of human cerebrocortical organoids. These organoids are differentiated from human patient-derived stem cells that bear a familial AD mutation in the presenilin 1 (PSEN1) gene, or from an isogenic wildtype (WT) control. The organoid conditioned media was aspirated from cultures and processed for EV enrichment using a non-invasive technique that requires no cellular disruption. EVs purified from AD organoid conditioned media have a wider size distribution and show differential expression of tetraspanins CD63, CD9, and CD81 when compared to WT organoid-derived EVs. AD organoid-derived EVs can have single, double, and even triple membranes and display luminal fibrillar material. A deep proteomic profiling of the EVs reveals several statistically significant differences, including evidence for modifications in secretory autophagy. EV isolates from both WT and AD organoids show strong binding to amyloid detecting dyes, both in bulk fluorescence and fluorescence microscopy assays. After a 1-week co-culture of AD organoids with WT organoids, there is evidence of endosomal membrane transfer between the isogenic cultures with an increase in amyloid-{beta} peptides in the WT organoids. These observations support the notion that non-cell-autonomous spread of amyloid-containing EVs in human AD brains can be modeled in a cerebral organoid system.

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Balistreri, A., Turner, N., Compher, J., Almaraz, M., Prabhavalkar, A., Chittal, S., Labra, S. R., Ezekiel, K., Baal, C., Cedeno Kwong, C., Ghatak, S., Schaefer, J.-H., Vanderpool, K., Spencer, K., Yates, J. R., Nolan, J. P., Henderson, S., Lipton, S. A., Kelly, J. W.. 2026-05-14. Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer's Disease Cerebrocortical Organoids. https://doi.org/10.64898/2026.05.13.724352

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