Genomic imprinting of the metabolic regulator gene Klf14 is regulated by a paternal sub-TAD anchored at Mest and a shared enhancer in mice.

Ha, A.; Hayashi, N.; Bogutz, A. B.; Moindrot, B.; Gomez, L.; Harris, J.; Marcil, A.; Court, F.; Shindo, M.; Arnaud, P.; Drouin, J.; Forne, T.; Noordermeer, D.; Takada, S.; Nakabayashi, K.; Lefebvre, L.

doi:10.64898/2026.05.09.724006

bioRxiv · 10.64898/2026.05.09.724006

Genomic imprinting of the metabolic regulator gene Klf14 is regulated by a paternal sub-TAD anchored at Mest and a shared enhancer in mice.

Ha, A.·Hayashi, N.·Bogutz, A. B.·Moindrot, B.·Gomez, L.·Harris, J.·Marcil, A.·Court, F.·Shindo, M.·Arnaud, P.·Drouin, J.·Forne, T.·Noordermeer, D.·Takada, S.·Nakabayashi, K.·Lefebvre, L.

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Abstract

KLF14 acts as a master regulator of gene expression in adipose tissue and variants at the human KLF14 gene show strong and reproducible association with type 2 diabetes and metabolic syndrome. Risk alleles are only pathologic when maternally inherited, consistent with the observation that KLF14/Klf14 is a maternally-expressed imprinted gene in human and mouse. However, how genomic imprinting is regulated at this important locus is currently unknown. In both species, KLF14/Klf14 is located [~]200 kb away from the paternally imprinted gene MEST/Mest, which is regulated by a maternal gametic differentially methylated region (gDMR) at its CpG island (CGI) promoter. Although the Klf14 CGI is kept unmethylated in most tissues, maternally-inherited DNA methylation (DNAme) marks are paradoxically required for Klf14 expression in mouse. Here, we show that Mest and Klf14 reside within the same topologically associating domain (TAD) in both mouse embryonic stem cells (ESCs) and differentiated cells, defined by sites of biallelic CTCF binding at the boundaries. Using allele-specific 4C-seq in F1 hybrid ESCs, we show that CTCF binding specifically to the unmethylated Mest gDMR generates a paternal allele-specific sub-TAD encompassing Klf14. We further show through CRISPR-Cas9-mediated mutagenesis, that deletions of the paternal Mest promoter region in ESCs as well as in vivo in mutant mice, results in both loss of Mest expression and acquisition of biallelic expression at Klf14. By analyzing epigenetic marks and chromatin looping in the Klf14-expressing pituitary cell line AtT-20, we identify a putative enhancer element shared by Mest and Klf14, providing a mechanistic model for the regulation of Klf14 imprinting. This work defines a new role for the Mest maternally methylated gDMR, revealing that it exerts long-range effects via allele-specific modulation of TAD structures and consequently act as an imprinting control region (ICR) in the regulation of Klf14 imprinting. Conservation of this TAD organisation at the human locus suggests that a similar regulatory mechanism operates at KLF14.

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Ha, A., Hayashi, N., Bogutz, A. B., Moindrot, B., Gomez, L., Harris, J., Marcil, A., Court, F., Shindo, M., Arnaud, P., Drouin, J., Forne, T., Noordermeer, D., Takada, S., Nakabayashi, K., Lefebvre, L.. 2026-05-13. Genomic imprinting of the metabolic regulator gene Klf14 is regulated by a paternal sub-TAD anchored at Mest and a shared enhancer in mice.. https://doi.org/10.64898/2026.05.09.724006

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