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bioRxiv · 10.64898/2026.05.08.723440

Manipulation of CA1 neuronal subtypes through Cre-mediated viral delivery in mice

Songara, D.·Ghosh, H. S.
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Abstract

CaMKII promoter is widely used to label and manipulate hippocampal pyramidal neurons via transgenic mouse lines or viral approaches. While it targets most excitatory neurons, a small subset remains unlabeled and often overlooked. We present an AAV-based strategy combined with CaMKII-driven Cre expression to access and study this remaining population. Furthermore, we provide a detailed protocol for in-house AAV production, targeted stereotaxic delivery, and functional validation of targeted neurons through slice electrophysiology and behavior. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=194 HEIGHT=200 SRC="FIGDIR/small/723440v1_ufig1.gif" ALT="Figure 1"> View larger version (50K): org.highwire.dtl.DTLVardef@3a31ccorg.highwire.dtl.DTLVardef@9b7e90org.highwire.dtl.DTLVardef@92297borg.highwire.dtl.DTLVardef@1e159eb_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Songara, D., Ghosh, H. S.. 2026-05-12. Manipulation of CA1 neuronal subtypes through Cre-mediated viral delivery in mice. https://doi.org/10.64898/2026.05.08.723440

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