Postprandial profiling of the duodenal microbiome reveals the impact of food structure and association with luminal metabolite and gut hormone responses

Warren, F.; Petropoulou, K.; Harris, H.; Barbas-Bernardos, C.; Kasapi, M.; Garcia, A.; Holmes, E.; Domoney, C.; Wist, J.; Garcia-Perez, I.; Frost, G.

doi:10.64898/2026.05.06.723166

bioRxiv · 10.64898/2026.05.06.723166

Postprandial profiling of the duodenal microbiome reveals the impact of food structure and association with luminal metabolite and gut hormone responses

Warren, F.·Petropoulou, K.·Harris, H.·Barbas-Bernardos, C.·Kasapi, M.·Garcia, A.·Holmes, E.·Domoney, C.·Wist, J.·Garcia-Perez, I.·Frost, G.

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Abstract

The human duodenum harbours a complex, dynamic microbial community that is challenging to study due to inaccessibility, particularly postprandially when nutrient-rich chyme and fluctuating metabolites create unique microbial niches. We used naso-duodenal intubation to longitudinally sample duodenal luminal contents following pea-based meals of differing food structure, alongside parallel blood collection. Shotgun metagenomic sequencing, comprehensive metabolomic profiling and gut hormone measurements were combined to explore microbe-metabolite-hormone interactions. Food structure significantly affected postprandial bacterial composition, with saccharolytic oral taxa increasing after meals with intact structure. Alpha diversity was influenced by structure type (P = 0.025), with whole pea seeds promoting greater diversity than pea flour. Network analysis revealed complex interactions between the duodenal microbiome, luminal metabolites and gut hormones, with most microbial associations linked to glucose-dependent insulinotropic polypeptide (GIP) rather than glucagon-like peptide-1 (GLP-1). Metabolic profiling showed meal-dependent changes in amino acid metabolism, including shifts in D/L amino acid ratios over time consistent with microbial metabolism. The duodenal microbiome showed close phylogenetic relationships with the oral microbiome, with composition influenced by food structuring and swallowing. These findings reveal dynamic microbe-metabolite interplay in the human duodenum during digestion and its relationship to gut hormone responses.

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Warren, F., Petropoulou, K., Harris, H., Barbas-Bernardos, C., Kasapi, M., Garcia, A., Holmes, E., Domoney, C., Wist, J., Garcia-Perez, I., Frost, G.. 2026-05-07. Postprandial profiling of the duodenal microbiome reveals the impact of food structure and association with luminal metabolite and gut hormone responses. https://doi.org/10.64898/2026.05.06.723166

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