Characterization of genetically effective cells and EMS mutagenesis on the novel winter oil seed Pennycress (Thlaspi arvense)

Brusa, A.; Branch, C.; Sulivan, L.; Chopra, R.; Rai, K.; Rockstad, G.; Gjesvold, E. S.; Ott, M.; Jain, S.; Biel, C. C.; Marks, M. D.

doi:10.64898/2026.04.30.722012

bioRxiv · 10.64898/2026.04.30.722012

Characterization of genetically effective cells and EMS mutagenesis on the novel winter oil seed Pennycress (Thlaspi arvense)

Brusa, A.·Branch, C.·Sulivan, L.·Chopra, R.·Rai, K.·Rockstad, G.·Gjesvold, E. S.·Ott, M.·Jain, S.·Biel, C. C.·Marks, M. D.

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Abstract

Pennycress (Thlaspi arvense L.) is an intermediate winter oilseed crop that has only recently been domesticated for agronomic use. Improving agronomic traits requires sources of genetic variation, and mutagenesis is frequently used to help overcome the limitations of natural populations. We investigate the impact of Ethyl methanesulfonate (EMS) on genetically effective cells (GECs) to characterize the intra-individual genetic variation of EMS mutagenesis in pennycress. We identified that pennycress contains at least 4 GECs which, when treated with EMS, create unique mutations across different branches within the same individual plant. We then propagated the M2 plants for whole genome sequencing, providing extensive characterization of the EMS mutation profile and developing a gene index as a resource for future reverse genetic screenings. Article SummaryPennycress is an emerging winter oil seed crop in the American Midwest. Domestication efforts have advanced rapidly through a combination of genetic techniques. One of the most successful methods has been the use of a mutant gene index, a large collection of pennycress seed where new genetic variation has been created through Ethyl methanesulfonate (EMS). EMS mutations are not uniform however, and a single treated seed can have wide genetic variation within the resulting plant. We investigate the role of genetically effective cells on EMS variation, and present the full EMS population as a resource for further pennycress domestication efforts.

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Brusa, A., Branch, C., Sulivan, L., Chopra, R., Rai, K., Rockstad, G., Gjesvold, E. S., Ott, M., Jain, S., Biel, C. C., Marks, M. D.. 2026-05-05. Characterization of genetically effective cells and EMS mutagenesis on the novel winter oil seed Pennycress (Thlaspi arvense). https://doi.org/10.64898/2026.04.30.722012

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