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bioRxiv · 10.64898/2026.04.30.721808

Simultaneous in vivo imaging of Ca2+ signals in periarteriolar cholinergic axonal varicosities and arteriole diameter changes in the mouse cerebral cortex

Watanabe, N.·Hotta, H.
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Abstract

Basal forebrain cholinergic neurons project widely to the cerebral cortex and participate in cerebrovascular regulation. Although cholinergic axons are distributed around the cerebrovasculature, their functional relationship with arteriolar dynamics remains unclear. In this study, we established an in vivo two-photon imaging approach to simultaneously measure Ca2+ signals in cholinergic axonal varicosities and arteriolar diameters in urethane-anesthetized mice. An adeno-associated virus (AAV) vector (rAAV-ChAT-jGCaMP8s) was injected into the nucleus basalis of Meynert. In vivo imaging of the frontal cortex revealed bead-shaped GCaMP signals around the arterioles. Pinch stimulation transiently increased Ca2+ signals in periarteriolar varicosities, followed by arteriolar dilation, with an approximately 2-s delay between their peaks. Linear regression analysis disclosed a significant relationship between the magnitudes of these changes. This approach enabled simultaneous evaluation of cholinergic axonal activity and arteriolar dynamics in vivo, providing a tool to investigate the cholinergic regulation of cerebrovasculature. HighlightsO_LIAAV-ChAT-GCaMP enables selective imaging of cholinergic projections C_LIO_LITwo-photon imaging reveals bead-shaped Ca2+ signals around arterioles C_LIO_LISensory stimulation increases periarteriolar cholinergic axonal Ca2+ signals C_LIO_LIAxonal Ca2+ signals are associated with arteriole dilation C_LI

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Watanabe, N., Hotta, H.. 2026-05-04. Simultaneous in vivo imaging of Ca2+ signals in periarteriolar cholinergic axonal varicosities and arteriole diameter changes in the mouse cerebral cortex. https://doi.org/10.64898/2026.04.30.721808

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