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bioRxiv · 10.64898/2026.04.29.721745

FastDedup - A fast and memory-efficient tool for read deduplication

Ribes, R.·Mandier, C.·Baniel, A.
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Abstract

PCR duplicate removal is a critical first step in high-throughput sequencing pipelines, yet existing tools struggle with speed, memory, or correctness at modern dataset scales. We present FastDedup, a Rust-based FASTX deduplicator that transforms each read or read pair to a compact xxh3 hash fingerprint, drastically reducing memory usage and binding most of the execution time to disk I/ O. Benchmarked against six competing tools on synthetic human WGS datasets up to 300 million reads, FastDedup consistently leads on paired-end data, running more than 10 times faster than fastp. It also outperforms all tools on uncompressed single-end data, deduplicating a million reads in a second. We additionally report correctness failures in prinseq++ and clumpify. FastDedup is available under the MIT License via GitHub, Bioconda, and Cargo.

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Ribes, R., Mandier, C., Baniel, A.. 2026-05-04. FastDedup - A fast and memory-efficient tool for read deduplication. https://doi.org/10.64898/2026.04.29.721745

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