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bioRxiv · 10.64898/2026.04.28.721496

Translation Inhibiting Antibiotics Induce a Strain Specific asRNA Regulating Purine Metabolism in S. pneumoniae TIGR4

Özkan, I.·van Opijnen, T.·Meyer, M. M.
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Abstract

Translation inhibiting antibiotics (TIA) are an important class of antibiotics that elicit a wide variety of transcriptional responses. In this work we characterize the transcriptional responses of Streptococcus pneumoniae TIGR4, via RNA-seq, 5-, and 3-end mapping, to sub-MIC levels of three TIA, chloramphenicol, tetracycline, and kasugamycin. We find that kasugamycin (initiation inhibitor) displays a distinct transcriptomic profile compared to chloramphenicol and tetracycline (elongation inhibitors). However, genes in nucleotide metabolism were consistently downregulated. We also detected a TIA induced antisense transcript complementary to downregulated genes for purine metabolism, which we term SP_0835as. Mutating the promoter for SP_0835as eliminates both induction of SP_0835as and repression of the complementary genes in response to chloramphenicol. The mutated strain also displays slower growth rates than control strains in conditions that strongly induce SP_0835as, implying biologically relevant activity. Furthermore, the SP_0835as promoter straddles the boundary between SP_0835 and an insertion sequence (ISL3) immediately downstream. A survey of S. pneumoniae genomes shows the promoter is present in several phylogenetically distinct strain clusters. However, other common laboratory strains of S. pneumoniae lack the promoter, SP_0835as expression, and TIA repression of the complementary genes. Thus, SP_0835as represents a nascent RNA regulator that significantly remodels the metabolic response to TIA.

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Özkan, I., van Opijnen, T., Meyer, M. M.. 2026-04-30. Translation Inhibiting Antibiotics Induce a Strain Specific asRNA Regulating Purine Metabolism in S. pneumoniae TIGR4. https://doi.org/10.64898/2026.04.28.721496

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