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bioRxiv · 10.64898/2026.04.23.720377

Connections across regional glymphatic clearance, neural activity and amyloid-β deposition in cortex

Li, Y.·Zhu, X.·zhou, y.·Zhang, X.·Zhou, Z.·Wei, K.·Sun, J.·Lou, M.
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Abstract

Neural activity inevitably produces waste, which promotes neurodegeneration with topographic features. The glymphatic system is important for waste clearance. However, the spatial characteristics of glymphatic clearance across cortex and whether it interplays with neural activity in contribution to amyloidosis in human remain unexplored. Here, by intrathecal administration of gadolinium-based contrast agents, glymphatic influx and clearance patterns across cortex in 96 participants are depicted via Glymphatic MRI. Analyses integrating post-mortem transcriptomic profiles from Allen Human Brain Atlas indicate that, genes related with excitatory and inhibitory neurons, and pathways engaging in synaptic function were enriched in regions with faster glymphatic clearance. FALFF was calculated from resting-state fMRI to represent neural activity. At the regional level, based on a subgroup with rs-fMRI (N = 15), regional glymphatic clearance was positively coupled with spontaneous neural activity. Mismatch index, reflecting decoupling between spontaneous neural activity and glymphatic clearance function, turned out to be positively associated with regional severity of amyloidosis using open-source 11C-PiB dataset. Together, this study for the first time demonstrates the intricate interplays between neural activity and glymphatic dynamics from transcriptional to physiological level. The mismatch between these two processes may serve as an undescribed comprehensive mechanism promoting regional vulnerability to proteopathy and subsequent neurodegeneration in cortex.

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Li, Y., Zhu, X., zhou, y., Zhang, X., Zhou, Z., Wei, K., Sun, J., Lou, M.. 2026-04-25. Connections across regional glymphatic clearance, neural activity and amyloid-β deposition in cortex. https://doi.org/10.64898/2026.04.23.720377

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