Ant abaecin-2 is a context-dependent copper-binding effector that can be either inhibitory or protective

Donaghy, C. M.; Heyer-Gray, H.; O'Hern, C.; Ibrahim, M.; Perez-Torres, B.; Bogues, N.; Alexandrescu, A.; Djoko, K.; Klassen, J. L.; Angeles-Boza, A. M.

doi:10.64898/2026.04.18.719391

bioRxiv · 10.64898/2026.04.18.719391

Ant abaecin-2 is a context-dependent copper-binding effector that can be either inhibitory or protective

Donaghy, C. M.·Heyer-Gray, H.·O'Hern, C.·Ibrahim, M.·Perez-Torres, B.·Bogues, N.·Alexandrescu, A.·Djoko, K.·Klassen, J. L.·Angeles-Boza, A. M.

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Abstract

Host defense peptides (HDPs) are important components of the innate immune system that are used to combat pathogens and often rely on metal binding for their function. However, controlling trace nutrients such as transition metals may have other roles in host-symbiont interactions beyond poisoning harmful pathogens. This study characterizes the evolution, structural properties, and biochemical activity of the novel hymenopteran HDP abaecin-2. In myrmicine ants such as the fungus-growing tribe Attini, abaecin-2 has evolved to include an Amino-Terminal Cu(II) and Ni(II)-binding (ATCUN) motif, which we hypothesize may bind copper, a trace nutrient that is enriched in attine ant colonies. Combined results from mass spectrometry, competitive binding assays, circular dichroism, and NMR indicate that the abaecin-2 peptide lacks a defined secondary structure and can associate with up to 2 Cu(II) ions, one strongly bound at the ATCUN motif and another weakly bound, likely at a conserved histidine residue. Despite its copper-binding activity, abaecin-2 alone does not exhibit antibacterial activity against Escherichia coli or Bacillus subtilis (models for bacteria that live in ant fungus gardens). However, it synergizes with a model pore-forming peptide cecropin A to inhibit the growth of E. coli, similar to the related peptide abaecin-1. The copper-binding activity conferred by the ATCUN motif also protects copper-sensitive E. coli from excess copper toxicity. The dual, context-dependent inhibitory and protective roles we propose for abaecin-2 indicate that this previously under-characterized HDP may be used by attine ants to regulate both harmful and beneficial symbionts. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=69 SRC="FIGDIR/small/719391v2_ufig1.gif" ALT="Figure 1"> View larger version (17K): org.highwire.dtl.DTLVardef@1298f41org.highwire.dtl.DTLVardef@17c16d9org.highwire.dtl.DTLVardef@1c03081org.highwire.dtl.DTLVardef@b0a5a6_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Donaghy, C. M., Heyer-Gray, H., O'Hern, C., Ibrahim, M., Perez-Torres, B., Bogues, N., Alexandrescu, A., Djoko, K., Klassen, J. L., Angeles-Boza, A. M.. 2026-04-21. Ant abaecin-2 is a context-dependent copper-binding effector that can be either inhibitory or protective. https://doi.org/10.64898/2026.04.18.719391

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