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bioRxiv · 10.64898/2026.04.15.718726

Droplet-compatible single-cell DNA methylation sequencing with PreTIC

Zhang, S.·Wang, F.·Zhang, Y.·Lee, K.-J.·Engman, C.·He, H.-Z.·Fan, Y.·Zheng, S.-Y.
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Abstract

Single-cell DNA methylation sequencing has remained technically specialized due to challenges of interfacing conversion chemistry with droplet microfluidics. We introduce pre-tagmentation in situ conversion (PreTIC), which enables whole-methylome profiling on a commercial droplet platform using off-the-shelf reagents. With PreTIC, we produce over 13,000 single-cell methylomes from fixed frozen cells in two days and resolve cell-specific epigenetic variation in human peripheral blood mononuclear cells.

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Zhang, S., Wang, F., Zhang, Y., Lee, K.-J., Engman, C., He, H.-Z., Fan, Y., Zheng, S.-Y.. 2026-04-18. Droplet-compatible single-cell DNA methylation sequencing with PreTIC. https://doi.org/10.64898/2026.04.15.718726

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