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bioRxiv · 10.64898/2026.03.31.715617

Draft Genome Sequence of Bacillus pergaminensis sp. nov. strain Bva_UNVM-123: A Promising Candidate for Bioremediation.

Peralta, C.·Sauka, D. H.·Felipe, V.·Del Valle, E. E.·Palma, L.
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Abstract

The Bacillus genus comprises physiologically versatile, endospore-forming bacteria widely distributed in natural environments. In this study, we report the isolation and genomic characterization of strain Bva_UNVM-123, recovered from agricultural soil in Pergamino, Argentina. Whole-genome sequencing using Illumina technology yielded a 5.1 Mbp draft genome assembled in 67 contigs with a GC content of 36%. Comparative genomic analyses using the TYGS server and digital DNA-DNA hybridization (dDDH) values supported its classification as a potentially novel species within the Bacillus sensu lato (s.l.) group. Genome annotation revealed 4,866 protein-coding genes, including multiple determinants conferring resistance to antibiotics (e.g., fosfomycin, tetracycline, beta-lactams) and toxic heavy metals (e.g., arsenic, cadmium, mercury), supporting its potential application in bioremediation. Additionally, PathogenFinder predicted a low probability of human pathogenicity (0.207), reinforcing its safety for environmental use. Functional classification based on Swiss-Prot further supported a metabolically versatile profile and revealed the presence of resistance-related categories associated with environmental adaptation. This study adds to the growing knowledge of environmental Bacillus species and their biotechnological potential.

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Peralta, C., Sauka, D. H., Felipe, V., Del Valle, E. E., Palma, L.. 2026-04-03. Draft Genome Sequence of Bacillus pergaminensis sp. nov. strain Bva_UNVM-123: A Promising Candidate for Bioremediation.. https://doi.org/10.64898/2026.03.31.715617

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