The in-vivo microstructural profile of human hippocampal subfield CA1 and its relation to memory performance

Hoepker Fernandes, J.; Hayek, D.; Vockert, N.; Garcia-Garcia, B.; Mattern, H.; Behrenbruch, N.; Fischer, L.; Kalyania, A.; Doehler, J.; Haemmerer, D.; Yi, Y.-Y.; Schreiber, S.; Maass, A.; Kuehn, E.

doi:10.64898/2026.03.30.714764

bioRxiv · 10.64898/2026.03.30.714764

The in-vivo microstructural profile of human hippocampal subfield CA1 and its relation to memory performance

Hoepker Fernandes, J.·Hayek, D.·Vockert, N.·Garcia-Garcia, B.·Mattern, H.·Behrenbruch, N.·Fischer, L.·Kalyania, A.·Doehler, J.·Haemmerer, D.·Yi, Y.-Y.·Schreiber, S.·Maass, A.·Kuehn, E.

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Abstract

The hippocampal CA1 subregion supports learning, memory formation, and spatial navigation. Although its three-layered architecture has been described in ex-vivo investigations, the in-vivo microstructural profile of CA1 and its relation to individual variations in memory performance remain poorly characterized. In this study, we used ultra-high field structural MRI at 7 Tesla to investigate the depth-dependent myelination patterns (measured by quantitative T1) of CA1 in younger adults, their relation to the local arterial architecture, and their association with individual differences in cognitive functions, specifically memory performance. Results show that left and right CA1 present depth-dependent patterns of myelination, with the outer and inner compartments showing higher myelination than the middle compartment. No significant relationship between layer-specific myelination of CA1 and distance to the nearest artery was observed. Right CA1 was found to be more myelinated than left CA1. Pairwise correlations and regression models showed that higher left CA1 myelination is linked to higher accuracy in object localization. Together, our data demonstrates the feasibility of describing the three layered myelin architecture of CA1 in vivo, and provides information on how alterations in the architecture of CA1 may relate to alterations in cognitive performance in younger adults.

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Hoepker Fernandes, J., Hayek, D., Vockert, N., Garcia-Garcia, B., Mattern, H., Behrenbruch, N., Fischer, L., Kalyania, A., Doehler, J., Haemmerer, D., Yi, Y.-Y., Schreiber, S., Maass, A., Kuehn, E.. 2026-04-01. The in-vivo microstructural profile of human hippocampal subfield CA1 and its relation to memory performance. https://doi.org/10.64898/2026.03.30.714764

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