Critical roles of MCM8 in meiotic recombination during mouse spermatogenesis

Surarapu, L. K.; Tilton, K.; Stritto, M. R. D.; Acharya, A.; Menendez, A. M.; Lu, M.; Shaheen, N.; Liang, S.; Iyer, M.; Cejka, P.; Pratto, F.; Jain, D.

doi:10.64898/2026.03.28.714908

bioRxiv · 10.64898/2026.03.28.714908

Critical roles of MCM8 in meiotic recombination during mouse spermatogenesis

Surarapu, L. K.·Tilton, K.·Stritto, M. R. D.·Acharya, A.·Menendez, A. M.·Lu, M.·Shaheen, N.·Liang, S.·Iyer, M.·Cejka, P.·Pratto, F.·Jain, D.

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Abstract

Meiotic DNA double-strand break (DSB) formation and repair by homologous recombination is crucial for ensuring proper chromosome segregation. In mice, the mini-chromosome maintenance family protein, MCM8, has been proposed to function in meiotic recombination and its loss leads to infertility, but the underlying mechanisms are poorly understood. Here we used cytological and genomic assays to infer the role of MCM8 during meiotic recombination in mouse spermatocytes. We show that MCM8-deficient spermatocytes exhibit increased levels of SPO11-dependent DSBs at recombination hotspots during early prophase. DSBs are resected normally and accumulate strand-exchange proteins. However, downstream recombination intermediates are barely detected and recombination intermediate-associated MutSgamma foci do not form efficiently. Consistent with a role in early recombination intermediate processing, MCM8 binds to displacement loop (D-loop) structures in vitro. We propose that MCM8 controls meiotic recombination in at least two ways. MCM8 participates in regulating meiotic DSB number. Further, MCM8 plays a role in the formation and/or stability of post-resection recombination intermediates, steps that are critical for DSB repair via recombination and for efficient synapsis of homologous chromosomes during mouse meiosis.

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Surarapu, L. K., Tilton, K., Stritto, M. R. D., Acharya, A., Menendez, A. M., Lu, M., Shaheen, N., Liang, S., Iyer, M., Cejka, P., Pratto, F., Jain, D.. 2026-03-30. Critical roles of MCM8 in meiotic recombination during mouse spermatogenesis. https://doi.org/10.64898/2026.03.28.714908

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