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bioRxiv · 10.64898/2026.03.16.711860

Protocol for calcium imaging of acute brain slices from Octopus vulgaris hatchlings during application of neurotransmitters

Courtney, A.·Van Dijck, M.·Styfhals, R.·Almansa, E.·Obenhaus, H. A.·Schafer, W. R.·Seuntjens, E.
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Abstract

Octopus vulgaris and other cephalopods are of increasing interest as neurobiological model organisms. This protocol describes a method to record calcium activity from individual cells in acute brain slices from Octopus vulgaris hatchlings during exogenous application of neurotransmitters. Using this protocol, we characterized single-cell responses to specific neurotransmitters in the optic lobes, which process visual information. The approach is readily adaptable to other cephalopods and small invertebrate species. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=146 HEIGHT=200 SRC="FIGDIR/small/711860v1_ufig1.gif" ALT="Figure 1"> View larger version (39K): org.highwire.dtl.DTLVardef@1a3da0forg.highwire.dtl.DTLVardef@412424org.highwire.dtl.DTLVardef@abda7eorg.highwire.dtl.DTLVardef@958ebf_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Courtney, A., Van Dijck, M., Styfhals, R., Almansa, E., Obenhaus, H. A., Schafer, W. R., Seuntjens, E.. 2026-03-18. Protocol for calcium imaging of acute brain slices from Octopus vulgaris hatchlings during application of neurotransmitters. https://doi.org/10.64898/2026.03.16.711860

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