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bioRxiv · 10.64898/2026.03.10.710607

Zasp52s differentially expressed intrinsically disordered region confers thin filament stability at the Z-disc

Ho, N.·Schöck, F.
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Abstract

The Drosophila scaffolding protein Zasp52 is required to maintain structure at the muscle Z-disc which experiences strong forces during contraction. It is alternatively spliced into many isoforms, some of which contain a long intrinsically disordered region (IDR). We show that this region is primarily expressed in the indirect flight muscle (IFM) and is required for maintaining integrity of the Z-disc. Deleting the IDR-encoding exon 15e results in flightlessness and structural IFM defects, including sarcomere bending at the Z-disc and an inability to de-contract. These defects are indicative of a lack of proper thin filament anchoring to the Z-disc. This is further supported by a genetic interaction between exon 15e and actin. Fluorescence recovery after photobleaching of an isoform lacking exon 15e shows that the IDR is required for maintaining Zasp52 at the Z-disc and thereby stabilizing Z-discs. Lastly, we can rescue these phenotypes by restricting IFM use. Together, these results suggest that Zasp52s IDR confers thin filament stability at the Z-disc of IFM.

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Ho, N., Schöck, F.. 2026-03-15. Zasp52s differentially expressed intrinsically disordered region confers thin filament stability at the Z-disc. https://doi.org/10.64898/2026.03.10.710607

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