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bioRxiv · 10.64898/2026.03.06.710201

Downregulation is the dominant effect of new regulatory mutations in a fungal pathogen

Sampaio, A. M.·Croll, D.
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Abstract

Well-tuned gene regulation is essential for an organisms survival. However, the origin and predominant effects of regulatory mutations remain poorly understood outside of model organisms. Here, we analyzed a large panel of genome sequencing data of the major fungal wheat pathogen Zymoseptoria tritici to recapitulate the evolutionary history of cis-regulatory mutations. We found that new mutations predominantly cause downregulation of the associated genes. The dominance of downregulation is reinforced for mutations occurring closest to the coding sequence and downstream. Mutations causing the strongest downregulation segregate at high frequencies in populations despite their recent origin. This suggests that selection may have played a role in their rapid increase since speciation. Overall, our study highlights the power of mapping populations combined with genomic surveys to unravel fundamental patterns of regulatory evolution.

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Sampaio, A. M., Croll, D.. 2026-03-10. Downregulation is the dominant effect of new regulatory mutations in a fungal pathogen. https://doi.org/10.64898/2026.03.06.710201

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