Asgard archaeal origin of microtubules

Löwe, J.; von Kügelgen, A.; Planelles-Herrero, V. J.; McAndrew, M. B. L.; Oliva, M. A.; Vosseberg, J.; Köstlbacher, S.; Dharamshi, J. E.; Appler, K. E.; MacLeod, F. I.; Nobs, S.-J.; Jorgensen, S. L.; Burns, B. P.; Baker, B. J.; Bharat, T. A. M.; Derivery, E.; Tamarit, D.; Ettema, T. J. G.

doi:10.64898/2026.02.15.705738

bioRxiv · 10.64898/2026.02.15.705738

Asgard archaeal origin of microtubules

Löwe, J.·von Kügelgen, A.·Planelles-Herrero, V. J.·McAndrew, M. B. L.·Oliva, M. A.·Vosseberg, J.·Köstlbacher, S.·Dharamshi, J. E.·Appler, K. E.·MacLeod, F. I.·Nobs, S.-J.·Jorgensen, S. L.·Burns, B. P.·Baker, B. J.·Bharat, T. A. M.·Derivery, E.·Tamarit, D.·Ettema, T. J. G.

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Abstract

Eukaryotic cells change their shapes, actively segregate their DNA and contain membrane networks, facilitated by a complex cytoskeleton containing actin filaments, microtubules made from tubulin, and other components. These filaments have ancient evolutionary origins since actin- and tubulin-like proteins form prokaryotic cytoskeletons in archaea and bacteria. Bona fide eukaryotic F-actin can be traced back to crenarchaea and Asgard archaea, which are the closest known relatives of eukaryotes. A possible Asgard archaeal origin of microtubules was suggested recently with the discovery of a lokiarchaeon containing AtubAB mini microtubules that share architectural features with their eukaryotic counterparts. Using phylogenetic analyses of metagenomic data, here we report the broad occurrence of tubulins in Asgard archaea. Biochemical and structural analyses showed that one of our newly discovered heimdallarchaeial AtubAB tubulin pairs forms four-protofilament mini-microtubules that show dynamic instability and are inhibited by the tubulin drug maytansine. Our work raises the possibility that microtubule architecture and dynamics evolved in Asgard archaea prior to eukaryogenesis.

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Löwe, J., von Kügelgen, A., Planelles-Herrero, V. J., McAndrew, M. B. L., Oliva, M. A., Vosseberg, J., Köstlbacher, S., Dharamshi, J. E., Appler, K. E., MacLeod, F. I., Nobs, S.-J., Jorgensen, S. L., Burns, B. P., Baker, B. J., Bharat, T. A. M., Derivery, E., Tamarit, D., Ettema, T. J. G.. 2026-02-16. Asgard archaeal origin of microtubules. https://doi.org/10.64898/2026.02.15.705738

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