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bioRxiv · 10.64898/2026.02.13.705774

Brainwide dopamine dynamics across sleep-wake transitions

Chen, C.·Tu, X.·Lu, L.·Pham, C.·Zhang, X.·Su, R.·Li, E.·Jin, Z.·Cao, W.·Yang, Y.·Kihiczak, M.·Hui, K.·Darmohray, D.
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Abstract

Dopaminergic signaling plays a critical role in regulating arousal and transitions between sleep states, yet brainwide dynamics underlying these transitions remain incompletely characterized. Here, we employed multi-site fiber photometry with the dopamine sensor GRAB-DA2m, combined with electroencephalogram (EEG)/electromyogram (EMG) recordings, to systematically assess regional dopamine (DA) dynamics across sleep-wake states in the medial prefrontal cortex (mPFC), striatal subregions, central amygdala (CeA), and midbrain nuclei in mice. We found that DA levels prominently increased in the mPFC, dorsolateral striatum (DLS), ventral tegmental area (VTA), substantia nigra pars compacta (SNc), and dorsal raphe nucleus (DRN) during transitions from non-rapid eye movement (NREM) sleep to wakefulness (WAKE), supporting their role in arousal. Conversely, DA decreased in the CeA and nucleus accumbens lateral parts (NAc-L) during these transitions. During transitions from NREM to rapid eye movement sleep (REM), DA elevations were observed in the CeA and middle accumbens subregion (NAc-M), rather than NAc-L, while other regions exhibited decreases. Cross-regional DA correlations revealed synchronized network activity across sleep-wake transitions. Optogenetic activation of VTA and DRN dopamine neurons induced robust DA release in cortical and subcortical regions, and chemogenetic activation promoted wakefulness selectively via VTA and DRN, but not SNc DA neurons. These results elucidate distinct brainwide DA dynamics across state transitions and highlight differential roles for DA signaling in modulating sleep-wake states.

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Chen, C., Tu, X., Lu, L., Pham, C., Zhang, X., Su, R., Li, E., Jin, Z., Cao, W., Yang, Y., Kihiczak, M., Hui, K., Darmohray, D.. 2026-02-16. Brainwide dopamine dynamics across sleep-wake transitions. https://doi.org/10.64898/2026.02.13.705774

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