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bioRxiv · 10.64898/2026.02.10.705206

Genetic variation shapes human mRNA translation and disease risk

Wang, S.·Chen, C.·Xiao, X.·Wang, Z.
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Abstract

Genetic variation can influence protein abundance through translation, yet this regulatory layer remains poorly defined. We developed a deep learning approach to systematically map the effects of single-nucleotide variant (SNV) on translation efficiency (TE) across the human genome. In lymphoblastoid cells, >90,000 variants substantially altered TE, with strong positional and sequence-context biases. Importantly, the missense variants, traditionally considered only for their effect on protein recoding, were also found to reshape translation efficiency, with proline substitutions consistently reducing TE in a length-dependent manner. Extending our analysis to eight additional cell types revealed a two-layer architecture of translational regulation: 5UTR variants produced highly concordant effects across cell types, whereas synonymous and missense variants in coding region exhibited cell type-specific outcomes, suggesting context-dependent translation regulation. TE-altering variants were enriched among GWAS loci and linked to cancer, immune, cardiometabolic, and neurological traits, positioning translation as a key mediator of genetic effects on disease.

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Wang, S., Chen, C., Xiao, X., Wang, Z.. 2026-02-12. Genetic variation shapes human mRNA translation and disease risk. https://doi.org/10.64898/2026.02.10.705206

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