Mutant ATXN1 impacts human and mouse microglia and contributes to cognitive, mood, and motor deficits in SCA1 mice

Selimovic, A.; Talukdar, G.; Fuchs, G.; Sharma, V.; Abbas, K. N.; Reddy, S. C.; Parnerkar, E.; Brooks, I. M.; Zhang, Y.; Koob, M.; Nakagawa, Y.; Orr, H.; Cvetanovic, M.

doi:10.64898/2026.02.10.705104

bioRxiv · 10.64898/2026.02.10.705104

Mutant ATXN1 impacts human and mouse microglia and contributes to cognitive, mood, and motor deficits in SCA1 mice

Selimovic, A.·Talukdar, G.·Fuchs, G.·Sharma, V.·Abbas, K. N.·Reddy, S. C.·Parnerkar, E.·Brooks, I. M.·Zhang, Y.·Koob, M.·Nakagawa, Y.·Orr, H.·Cvetanovic, M.

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Abstract

Microglia, resident immune cells of the brain, are important players in neurodegeneration. While microglial activation is a hallmark of many neurodegenerative diseases, the specific role of microglia intrinsic factors in microglial activation and disease pathogenesis remains unknown. Spinocerebellar ataxia type-1 (SCA1) is an inherited autosomal dominant neurodegenerative disease characterized by severe neuronal loss and early microglial activation in the cerebellum. SCA1 is caused by CAG repeat expansion in the ubiquitously expressed ATAXIN1 (ATXN1) gene. Using human microglia differentiated from SCA1 patient derived iPSCs, we found that mutant ATXN1 is sufficient to alter morphology, gene and protein expression in human microglia in a cell-autonomous manner. Moreover, compared to controls, human SCA1 microglia exhibited increased phagocytosis and pro-inflammatory cytokine production, indicating an immune priming. To determine the extent to which mutant ATXN1 in microglia contributes to SCA1 pathogenesis and behavioral symptoms, we removed mutant ATXN1 from microglia and macrophages in a novel conditional SCA1 mouse model, f-ATXN1146Q/2Q mice. Microglial mutant ATXN1 reduction led to a marked correction in microglia phenotype, in particular in the transcriptomic signature of interferon type 1 mediated immune response, reduced microglial density and resulted in smaller microglia with reduced branching in the cerebellum. Pathology of Purkinje neurons and cerebellar astrogliosis were also ameliorated. Utilizing a battery of behavioral tests, we found that microglia and macrophage mutant ATXN1 reduction ameliorated cognitive, mood, and motor deficits in SCA1 mice. Together, these results indicate that mutant ATXN1 directly impacts microglial phenotype in SCA1, contributing to SCA1 pathology and behavioral deficits.

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Selimovic, A., Talukdar, G., Fuchs, G., Sharma, V., Abbas, K. N., Reddy, S. C., Parnerkar, E., Brooks, I. M., Zhang, Y., Koob, M., Nakagawa, Y., Orr, H., Cvetanovic, M.. 2026-02-12. Mutant ATXN1 impacts human and mouse microglia and contributes to cognitive, mood, and motor deficits in SCA1 mice. https://doi.org/10.64898/2026.02.10.705104

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