Effects of microgravity on the three-dimensional morphology of rhizoids in Physcomitrium patens

Abstract

Rooting systems of plants perceive environmental stimuli and flexibly regulate their growth. Therefore, understanding stimulus perception and response mechanisms is essential for optimizing cultivation. During the transition from aquatic to terrestrial environments, land plants have acquired mechanisms to adapt to gravitational force on land. Thus, elucidating gravity responses of rhizoids in bryophytes, early diverging land plants, provides important insights into how gravity-response mechanisms were established during land plant evolution. Analyzing rhizoid morphology under microgravity, where gravitational effects are largely eliminated, provides an effective approach to examine the gravity-response mechanisms that evolved after terrestrialization. In this study, to elucidate microgravity effects on rhizoid growth of Physcomitrium patens, we analyzed 3D datasets obtained by refraction-contrast micro-CT using synchrotron radiation after fixation and embedding of samples from the Space Moss experiment conducted on the International Space Station. Because each CT volume contains numerous rhizoids, we optimized a WEKA-based machine-learning segmentation approach by improving preprocessing, training, and postprocessing steps, resulting in a significantly improved segmentation accuracy. Comparison of 3D morphological indices between manually segmented rhizoids and predicted results supported the validity of the proposed method for morphological analysis. Morphological analyses revealed that, compared with both ground and artificial 1 x g conditions, rhizoid elongation and gravitropic responses were suppressed under microgravity, leading to reduced vertical growth. These findings indicate that gravity plays a fundamental role in rhizoid morphogenesis, and their absence affects growth orientation and elongation. This study provides foundational data for research on the rooting systems of bryophytes in space.