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bioRxiv · 10.64898/2026.01.29.702655

Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques

Li, Z.·Dicks, M. D. J.·Rose, L. M.·Russell, R. A.·McMahan, K.·Lasrado, N.·Nkolola, J.·Borducchi, E.·Liu, J.·Biswas, S.·Barouch, D. H.
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Abstract

Adenovirus vector-based vaccines were deployed widely during the COVID-19 pandemic. In this study, we explored the potential of displaying an antigen on the surface of the adenovirus capsid as well as encoding an antigen as a transgene in the adenovirus vector to optimize both humoral and cellular immune responses. We show that displaying SARS-CoV-2 Spike receptor biding domain (RBD) on the Ad5 capsid while simultaneously encoding Spike as a transgene induced robust antibody and T cell responses in rhesus macaques. These data demonstrate that adenoviruses can be utilized simultaneously as both nanoparticle scaffolds and viral vectors.

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Li, Z., Dicks, M. D. J., Rose, L. M., Russell, R. A., McMahan, K., Lasrado, N., Nkolola, J., Borducchi, E., Liu, J., Biswas, S., Barouch, D. H.. 2026-01-30. Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques. https://doi.org/10.64898/2026.01.29.702655

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