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bioRxiv · 10.64898/2026.01.27.700572

Live dynamics of induced cell-cell fusion between mitotic and interphasic cells

Afonso, O.·Feliciano, D.·Lippincott-Schwartz, J.
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Abstract

The cell cycle is tightly regulated by checkpoint mechanisms that ensure faithful duplication and segregation of the genome. Here, we induced cell-cell fusion between mitotic and interphase cells to study how nuclei from different cell cycle stages behave in a shared cytoplasm. We found that mitosis is a dominant cell cycle state: the mitotic cytoplasm can drive interphase nuclei into mitosis, whereas, in high ratios of interphase versus mitotic nuclei, fusion forced mitotic nuclei to exit mitosis. Both outcomes represent checkpoint override events with impactful consequences. Interphase nuclei forced into mitosis form aberrant mitotic spindles, show partially condensed DNA and ultimately undergo mitotic catastrophe. Conversely, forced mitotic exit resulted in reformation of nuclear envelope membranes around condensed chromosomes, forming nuclei with a defective nuclear import machinery. Altogether, cell-cell fusion revealed an unexpected plasticity in cell cycle control and highlight cell-cell fusion experiments as a powerful experimental system to study how competing cytoplasmic states are integrated in a shared cytoplasm.

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Afonso, O., Feliciano, D., Lippincott-Schwartz, J.. 2026-01-27. Live dynamics of induced cell-cell fusion between mitotic and interphasic cells. https://doi.org/10.64898/2026.01.27.700572

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