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bioRxiv · 10.64898/2026.01.20.700739

AI-designed nuclease performs robust knock out, base editing and prime editing in plants

Das, P.·Saha, R.·Panda, D.·Ghosh, C.·Avinash, S. P.·Panda, S.·Baig, M. J.·Molla, K. A.
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Abstract

Genome-editing applications in biological organisms rely on a limited set of naturally occurring enzymes. Artificial intelligence (AI) is transforming protein design. Here, building on the AI-designed nuclease OpenCRISPR-1, we establish the plant-optimized AI-designed editors (PAiD) as a versatile genome-editing platform in plants. PAiD supports NHEJ-mediated indel generation, adenine and cytosine base editing, and prime editing, with robust performance across multiple loci comparable to SpCas9. These results highlight the translational potential of AI-designed nucleases for plant genome engineering.

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Das, P., Saha, R., Panda, D., Ghosh, C., Avinash, S. P., Panda, S., Baig, M. J., Molla, K. A.. 2026-01-22. AI-designed nuclease performs robust knock out, base editing and prime editing in plants. https://doi.org/10.64898/2026.01.20.700739

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