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bioRxiv · 10.64898/2026.01.13.699341

Hypatia: a set of quantitative methods for profiling isoform across cell populations

Pan, T.·Shiau, C.-K. S.·Lu, L.·Wang, C.·Wang, M.·He, Y.·Bhimaraj, A.·Brat, D. J.·Huse, J. T.·Li, J. J.·Gao, R.
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Abstract

High-throughput long-read single-cell RNA-sequencing enables isoform-level study across single cells, yet methods for systematically assessing cell-to-cell variations remain limited. Here, we develop Hypatia, a comprehensive platform for dissecting isoform complexities across cell populations, devising Tsallis entropy and Cramers V to facilitate robust comparative profiling. Hypatia revealed prominent isoform species variations and usage shifts across cell-types in glioblastoma, renal cell carcinoma, and heart, highlighting clinically relevant applications for studying isoform-derived, cell-specific functions.

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Pan, T., Shiau, C.-K. S., Lu, L., Wang, C., Wang, M., He, Y., Bhimaraj, A., Brat, D. J., Huse, J. T., Li, J. J., Gao, R.. 2026-01-14. Hypatia: a set of quantitative methods for profiling isoform across cell populations. https://doi.org/10.64898/2026.01.13.699341

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