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bioRxiv · 10.64898/2026.01.12.699156

Microglia Piezo1 aggravates cerebral ischemia and reperfusion injury by chemotactic recruitment of T lymphocytes

Zhang, H.-N.·Chang, H.-M.·Wang, B.·Gao, T.·Zhang, H.·Xu, S.-J.·Wang, X.·Liu, F.·Huang, J.
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Abstract

Cerebral ischemia reperfusion injury (CIRI) is a complex pathophysiological process involving multiple mechanisms. Piezo1, a stretch-activated ion channel, has recently emerged as a potential regulator of cellular responses to ischemic conditions. However, its role in specific cells during ischemic events is not well elucidated. Here, we showed that after experimentally induced CIRI, Piezo1 channel was highly expressed in peri-infarct area, where is upregulated and activated mainly in microglia. Behavioral tests, and infarct volume measurements demonstrated that conditional Piezo1 deficient in microglia markedly ameliorated the neurological deficits and reduced the infarct volumes. Flow cytometry and immunofluorescence staining showed decreased peripheral immune cells, especially T lymphocytes infiltration into the brain in microglia Piezo1 deficient mice after stroke. Multiplex chemokine immunoassay and T cell migration assays screened that Piezo1 deficient microglia blocked the CXC chemokine ligand 10 (CXCL10) release from astrocyte, which lead to the inhibited T cell recruitment in transwell co-cultured system. Furthermore, RNA-Sequencing analysis showed that defective Piezo1 in microglia lead to its polarization towards an anti-inflammatory phenotype in response to cerebral ischemia and reduced the secretion of tumor necrosis factor- (TNF-) and interferon-{gamma} (IFN-{gamma}). Collectively, Piezo1 deficiency inhibited the secretion of TNF- and IFN-{gamma} in microglia. The reduction of these inflammatory cytokines further blocked the CXCL10 release from astrocyte, and ultimately ameliorated the infiltration of peripheral T lymphocytes after stroke. Our results therefore highlight the critical role of Piezo1 in microglia-orchestrated neuroinflammation and suggest a potential means for reducing stroke-induced neurological injury.

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Zhang, H.-N., Chang, H.-M., Wang, B., Gao, T., Zhang, H., Xu, S.-J., Wang, X., Liu, F., Huang, J.. 2026-01-13. Microglia Piezo1 aggravates cerebral ischemia and reperfusion injury by chemotactic recruitment of T lymphocytes. https://doi.org/10.64898/2026.01.12.699156

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