Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons

Abstract

The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules are maintained and renewed in the face of this dynamic endeavor and long-term commitment remains unclear. Recently, in-lattice repair has emerged as an alternative mechanism of microtubule renewal that could allow for continuity of the existing network and for the emergence of cell polarity. Here, we directly assessed microtubule turnover using microinjection of labeled tubulin in cultured hippocampal neurons that exhibit well defined stages of development and polarization during the first 24 hours in culture. Sizeable tubulin integration was visible minutes after microinjection, indicating fast turnover of microtubules in developing neurons. Once it appeared, a longer neurite that would become the nascent axon showed decreased turnover, both for its acetylated and non-acetylated populations of microtubules. Combining microinjection with expansion microscopy allowed us to visualize tubulin integration sites along single microtubules, unambiguously demonstrating the existence of in-lattice integration along neurites. In thick neurites, we observed preferential integration within non-acetylated cortical microtubules, but in-lattice integration sites were also visible in the deeper core bundles of acetylated microtubules. Overall, our results link previous observations of microtubule stabilization patterns in developing neurons to their actual turnover. Mapping these patterns of turnover strengthens the notion that microtubules establish an organized network that participates in axon emergence and the establishment of neuronal polarity.