Isothermal Detection of Influenza D using RT-LAMP

dos Santos, C. A.; Li, J.; van Diemen, P. M.; McMahon, A.; Mollett, B. C.; Ramsay, A. M.; Maina, M. M.; James, J.; Everett, H. E.; Daly, J. M.; Robb, N. C.

doi:10.64898/2026.01.09.698603

bioRxiv · 10.64898/2026.01.09.698603

Isothermal Detection of Influenza D using RT-LAMP

dos Santos, C. A.·Li, J.·van Diemen, P. M.·McMahon, A.·Mollett, B. C.·Ramsay, A. M.·Maina, M. M.·James, J.·Everett, H. E.·Daly, J. M.·Robb, N. C.

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Abstract

The Orthomyxoviridae family includes influenza D virus (IDV), an emerging pathogen primarily affecting cattle and swine, with evidence of cross-species transmission and potential zoonotic risk. Although active human infections have yet to been confirmed, high seroprevalence in cattle-exposed populations highlights the need for continued surveillance. Here, a rapid, field-deployable RT-LAMP assay for IDV detection was developed and validated, with 99.2% specificity and sensitivity ranging from 95.6% (Cq < 30) to 81.8% (Cq < 40). This method offers a cost-effective, accessible alternative to RT-qPCR, enabling improved monitoring of IDV, and reinforcing preparedness for emerging influenza threats.

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dos Santos, C. A., Li, J., van Diemen, P. M., McMahon, A., Mollett, B. C., Ramsay, A. M., Maina, M. M., James, J., Everett, H. E., Daly, J. M., Robb, N. C.. 2026-01-09. Isothermal Detection of Influenza D using RT-LAMP. https://doi.org/10.64898/2026.01.09.698603

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