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bioRxiv · 10.64898/2026.01.06.698063

FUSION: A fast and uniform processing framework for whole-brain optical scattering tomography images

CHEN, C.·GU, P.·VILLIGER, M.·URIBE-PATARROYO, N.·BOUMA, B.·REN, J.
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Abstract

Coherent scattering imaging has seen substantial development in clinical ophthalmology and endoscopic microscopy owing to its high throughput, large dynamic range, and capability for label-free imaging. However, limited optical penetration has constrained its application to whole-organ imaging studies. Here, we propose a high-throughput coherent scattering imaging system assisted by tissue clearing, termed fuCAST, which enables whole-brain white-matter imaging. In parallel, we develop a high-throughput image reconstruction and uniform stitching platform, FUSION, specifically tailored for fuCAST datasets.

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CHEN, C., GU, P., VILLIGER, M., URIBE-PATARROYO, N., BOUMA, B., REN, J.. 2026-01-07. FUSION: A fast and uniform processing framework for whole-brain optical scattering tomography images. https://doi.org/10.64898/2026.01.06.698063

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