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bioRxiv · 10.64898/2026.01.06.697137

Cellular basis of accelerated whole-tooth regeneration

Mubeen, T.·He, H.·Gruenhagen, G.·Satoskar, A.·Streelman, J. T.
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Abstract

Teeth are ectodermal organs that have, throughout their long evolutionary history, retained the capacity for full regeneration and replacement, even in adult stages. Yet, because most mammals (e.g., humans, mice) lack lifelong dental replacement, we do not fully understand its tempo and mode, and we do not have a clear picture of the cell populations and signals that contribute to the process. Here, we used cichlid fishes from Lake Malawi, species that differ in tooth formula (tooth shape and number) but share one-for-one tooth replacement, to (i) explore the tempo of dental replacement after plucking and then (ii) identify the cell populations, gene expression signatures, and interactions between cell populations that change in this plucking paradigm. We observed that cichlid species with divergent dentitions accelerated tooth replacement >3x on the plucked half of the jaw. Then, we used single-nucleus RNA-seq to profile cellular and molecular changes across the first week of post-plucking tooth replacement. This approach allowed us to infer cellular trajectories in dental epithelium and mesenchyme that underlie tooth regeneration. We identifed distinct gene expression profiles and cellular interactions across four time points of accelerated tooth replacement, with divergent involvement of epithelial, mesenchymal and immune cell types. Diferential signaling of Collagen, BMP, MMP, Semaphorin and Slit-Robo pathways was evident after plucking and highlights temporally-sequenced roles of immune response, odontogenesis, vascularization and nerve pathfinding as teeth are constructed anew. Overall, this study provides insight into the trajectory of cellular interactions accompanying whole-tooth replacement and offers a comparative foundation for understanding dental regeneration in vertebrates.

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Mubeen, T., He, H., Gruenhagen, G., Satoskar, A., Streelman, J. T.. 2026-01-07. Cellular basis of accelerated whole-tooth regeneration. https://doi.org/10.64898/2026.01.06.697137

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