Minimally Invasive in Situ Perfusion Method for Targeted AAV Delivery in Native Kidney: Proof of Concept in Pigs

Montagud-Marrahi, E.; Banon-Maneus, E.; Zugazaga, A.; Gutierrez, D.; Zattera, L.; Rabadan-Ros, R.; Lazo, M.; Bohils, M.; Gelabert, A.; LUQUE RINCON, Y.

doi:10.64898/2026.01.03.697116

bioRxiv · 10.64898/2026.01.03.697116

Minimally Invasive in Situ Perfusion Method for Targeted AAV Delivery in Native Kidney: Proof of Concept in Pigs

Montagud-Marrahi, E.·Banon-Maneus, E.·Zugazaga, A.·Gutierrez, D.·Zattera, L.·Rabadan-Ros, R.·Lazo, M.·Bohils, M.·Gelabert, A.·LUQUE RINCON, Y.

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Abstract

Currently, there is not any method for efficient and targeted delivery of gene therapies using viral vectors to the native kidneys. Adeno-associated viruses (AAV) are limited by hepatotoxicity and poor kidney tropism. This study aimed to develop a minimally invasive approach that overcomes both challenges. We evaluated in pigs a technique leveraging external perfusion systems to transiently isolate native kidney vascularization. Using a percutaneous femoral approach, fluoroscopy-guided catheterization of the kidney artery and vein enabled the establishment of a temporary isolated kidney perfusion circuit permitting native kidney in-situ perfusion. AAV delivery was then assessed by detecting construct encoding Td-Tomato protein, its mRNA and fluorescence 6 days after the perfusion. The method was achieved with two different external perfusion systems and was not associated with procedure related mortality or any major complication. Td-Tomato was detected in the perfused kidney without a significant detection in the liver which showed no inflammatory infiltrates. We developed, in pigs, a minimally invasive method permitting in-situ native kidney perfusion that enables kidney-targeted AAV delivery. Besides, it limits liver off-target transduction, potentially reducing its main side effect, hepatotoxicity, when AAV is systemically injected. It offers fast-track translational possibilities for developing kidney-targeted gene therapies. Finally, it may permit additional non-AAV therapeutics for patients with kidney diseases.

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Montagud-Marrahi, E., Banon-Maneus, E., Zugazaga, A., Gutierrez, D., Zattera, L., Rabadan-Ros, R., Lazo, M., Bohils, M., Gelabert, A., LUQUE RINCON, Y.. 2026-01-03. Minimally Invasive in Situ Perfusion Method for Targeted AAV Delivery in Native Kidney: Proof of Concept in Pigs. https://doi.org/10.64898/2026.01.03.697116

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