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bioRxiv · 10.64898/2025.12.29.696956

Yeast as a platform to dissect poly(ADP-ribose) polymerase function from Magnaporthe oryzae and evaluate PARP inhibitors

Kalicharan, R.·Paynes, N.·Fernandez, J.
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Abstract

Poly(ADP-ribose) polymerases (PARPs) regulate genome maintenance through NAD+-dependent ADP-ribosylation, yet PARP function in fungi remains poorly defined. Here, we reconstituted the activity of the Magnaporthe oryzae PARP1 homolog (MoPARP1) in Saccharomyces cerevisiae, a genetically tractable organism that lacks endogenous PARP enzymes. Galactose-induced expression of MoPARP1 reduced yeast growth, whereas a catalytically inactive mutant showed no defect, indicating that the growth phenotype depends on PARP catalytic activity. In a multidrug transporter-deficient background, the PARP inhibitor 3-aminobenzamide and the clinically used PARP inhibitor olaparib rescued the growth of MoPARP1-expressing strains, establishing a framework for inhibitor testing in vivo. Finally, MoPARP1-GFP localized to the nucleus independent of catalytic activity, supporting correct targeting in this heterologous system. Together, these findings establish yeast as a platform to dissect fungal PARP biology and evaluate chemical inhibition.

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Kalicharan, R., Paynes, N., Fernandez, J.. 2025-12-30. Yeast as a platform to dissect poly(ADP-ribose) polymerase function from Magnaporthe oryzae and evaluate PARP inhibitors. https://doi.org/10.64898/2025.12.29.696956

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