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bioRxiv · 10.64898/2025.12.22.695898

Antibody-based signal amplification for single-cell proteomics by mass spectrometry

Woessmann, J.·Agullet, J. P.·Blomberg, A. L.·Goletz, S.·Porse, B. T.·Schoof, E. M.
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Abstract

Single-cell proteomics by mass spectrometry (scp-MS) detects thousands of proteins per cell, yet low-abundant proteins routinely detected by antibody-based methods are challenging to quantify in the single-cell proteome. To address this, we developed antibody peptide tags (AbTags) - MS detectable tandem peptide barcodes coupled to antibodies which provide signal amplification, enable absolute quantification, and are quantified alongside endogenous proteomes by scp-MS. As proof-of-concept, AbTags quantify cytokine-induced PDL1 expression, undetectable by scp-MS.

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Woessmann, J., Agullet, J. P., Blomberg, A. L., Goletz, S., Porse, B. T., Schoof, E. M.. 2025-12-24. Antibody-based signal amplification for single-cell proteomics by mass spectrometry. https://doi.org/10.64898/2025.12.22.695898

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