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bioRxiv · 10.64898/2025.12.22.695889

Quantitative analysis of synaptic zinc in the brain by covalent chemistry-based semisynthetic biosensors

Zhu, H.·Sakamoto, S.·Nakamura, K.·Chengli, Q.·Nakajima, K.·Nonaka, H.·Hamachi, I.
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Abstract

Labile zinc ions (Zn{superscript 2}) are stored in glutamatergic vesicles at specific excitatory synapses in the central nervous system and released into synaptic clefts in an activity-dependent manner. Although Zn{superscript 2} is suggested to modulate various neuroreceptor functions, its precise roles remain unclear due to a lack of tools capable of quantitatively analyzing Zn{superscript 2} with synapse-level spatial resolution. Here, we developed neuroreceptor-based semisynthetic sensors that record synaptic Zn{superscript 2} dynamics by covalent chemistry in the living mouse brain. Using a chemical knock-in strategy, we introduced activity-based Zn2+-probes with distinct affinities into endogenous -amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid or {gamma}-aminobutyric acid type A receptors. These in-brain-constructed Zn{superscript 2} sensors enabled, to our knowledge, for the first time, quantitative, region-specific mapping of Zn{superscript 2} released into synaptic clefts. Imaging-based analyses revealed differences in Zn{superscript 2} concentrations at excitatory and inhibitory synapses across hippocampal regions during kainate-induced seizures, providing new insights into the physiological and pathological functions of synaptic Zn{superscript 2}.

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Zhu, H., Sakamoto, S., Nakamura, K., Chengli, Q., Nakajima, K., Nonaka, H., Hamachi, I.. 2025-12-23. Quantitative analysis of synaptic zinc in the brain by covalent chemistry-based semisynthetic biosensors. https://doi.org/10.64898/2025.12.22.695889

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