Neocortical temporal patterning by a two-layered regulatory network

Yuan, G.; Zhao, Z.; Dong, X.; Wang, X.-W.; Zhang, Q.; Yu, X.; Zhu, M.; Yu, J.; Zhang, D.; Zhang, N.; Xu, Z.; Dai, L.; Liu, Y.-Y.; Shi, S.-H.; Li, Y.

doi:10.64898/2025.12.18.695250

bioRxiv · 10.64898/2025.12.18.695250

Neocortical temporal patterning by a two-layered regulatory network

Yuan, G.·Zhao, Z.·Dong, X.·Wang, X.-W.·Zhang, Q.·Yu, X.·Zhu, M.·Yu, J.·Zhang, D.·Zhang, N.·Xu, Z.·Dai, L.·Liu, Y.-Y.·Shi, S.-H.·Li, Y.

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Abstract

In the developing neocortex, a diverse array of neurons with defined types and abundances are systematically generated by a limited population of radial glial progenitors (RGPs) as they undergo successive fate changes. The molecular regulation behind this intricate temporal patterning remains elusive. We undertook in-depth single-cell multi-omics analyses, discovering a two-layered regulatory framework at the core of this process. Central to this are global temporal regulators positioned above a temporal network, consisting of series of transcriptional factor (TF) hub groups under sequential state transitions. This temporal network operates not by restricting TF expressions to discrete temporal windows, but through coordinated transcriptional and chromatin-accessibility dynamics that modulate transient TF regulatory activity. Moreover, global temporal regulators specify the duration of each cascading stage and, consequently, the number of progenies generated at each stage. Loss of global temporal regulators protracts RGP lineage progression, whereas their increased activity accelerates it. These findings suggest a two-layer temporal regulatory system controlling RGP lineage progression and neural progeny output duality in mammalian neocortical development.

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Yuan, G., Zhao, Z., Dong, X., Wang, X.-W., Zhang, Q., Yu, X., Zhu, M., Yu, J., Zhang, D., Zhang, N., Xu, Z., Dai, L., Liu, Y.-Y., Shi, S.-H., Li, Y.. 2025-12-22. Neocortical temporal patterning by a two-layered regulatory network. https://doi.org/10.64898/2025.12.18.695250

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