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bioRxiv · 10.64898/2025.12.17.694872

Aromatic lactic acid production by Bifidobacterium longum subsp. infantis is determined by cell density, substrate availability and pH in vitro

Plenge, T. G.·Pedersen, M.·Laursen, M. F.
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Abstract

Bifidobacterium longum subsp. infantis (B. infantis) is an important colonizer of the infant gut. This species is known to produce a variety of health beneficial metabolites, including aromatic lactic acids (ALAs), supporting early life immune development. However, the regulation of ALA production in B. infantis remains poorly understood. In this study, we investigated how environmental factors, such as substrate availability and pH affect B. infantis DSM20088 growth and ALA production in vitro. Bacterial batch cultivations supplemented with different amino acid concentrations revealed a linear relationship between indole-3-lactic acid (ILA), 4-hydroxyphenyllactic acid (OH-PLA), and phenyllactic acid (PLA) production and exogenous concentrations of tryptophan, tyrosine and phenylalanine, respectively. Furthermore, chemostat cultivations at physiological relevant pH levels (4.5, 5.5 and 6.5) showed that an acidic pH slowed growth and shifted ALA production by B. infantis. Overall, ALA concentrations correlated strongly with bacterial abundance across and within pH levels, showing that cell density is a major determinant of ALA production. After considering the growth limiting effects of lower environmental pH, it had limited impact on the total production of ALAs, but the relative production of PLA was stepwise enhanced at more acidic pH at the expense of ILA, independent of cell density. In conclusion, this study shows that cell density, substrate availability and environmental pH determine aromatic lactic acid production by B. infantis. ImportanceBifidobacterium longum subsp. infantis is an abundant bacterial species in the infant gut microbiota where it supports immune development e.g. though the production of aromatic lactic acids. In this study we cultured Bifidobacterium longum subsp. infantis at different concentrations of aromatic amino acids and pH levels to investigate the effect of these environmental factors on aromatic lactic acid production. We show that there is a linear relationship between aromatic lactic acid production and the exogenous aromatic amino acid concentration, that the production strongly follows cell density, and that pH regulates the preference towards producing different aromatic lactic acids. This knowledge may help inform strategies to enhance or direct beneficial ALA production in the infant gut.

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Plenge, T. G., Pedersen, M., Laursen, M. F.. 2025-12-18. Aromatic lactic acid production by Bifidobacterium longum subsp. infantis is determined by cell density, substrate availability and pH in vitro. https://doi.org/10.64898/2025.12.17.694872

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