Mapping glioblastoma spreading: connexin43 and glial dynamic in mouse and human glioblastoma microenvironment

Virtuoso, A.; Milior, G.; De Luca, C.; Moulard, J.; Riccio, L.; Evstratova, A.; Venard, A.; Cirillo, G.; Pallud, J.; Huberfeld, G.; Rouach, N.; Papa, M.

doi:10.64898/2025.12.16.694626

bioRxiv · 10.64898/2025.12.16.694626

Mapping glioblastoma spreading: connexin43 and glial dynamic in mouse and human glioblastoma microenvironment

Virtuoso, A.·Milior, G.·De Luca, C.·Moulard, J.·Riccio, L.·Evstratova, A.·Venard, A.·Cirillo, G.·Pallud, J.·Huberfeld, G.·Rouach, N.·Papa, M.

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Abstract

High-grade gliomas (HGGs), including astrocytoma and glioblastoma (GBM), constitute the most prevalent primary tumors of the central nervous system (CNS). GBM cells demonstrate a notable ability to infiltrate the brain parenchyma, precluding complete surgical resection. Here we investigated the spreading of GBM cells and the response of the CNS microenvironment focusing on glial cells, which are essential interactors to GBM. We used acute and organotypic slices from the mouse brain and peritumoral cortex of patients with HGGs. We found that human peritumoral tissue from cortical resection was characterized by high levels of the astrocytic Connexin43 protein (Cx43) and discrete infiltration of microglia. In contrast, the tumor core exhibited high myeloid infiltration and an altered extracellular matrix (ECM) composition, which was poor in CD44. We tracked mouse and primary human-labeled-GBM cells in 2D cultures and in co-culture with organotypic slices generated from mouse brain and human peritumoral tissues. We found that the implanted GBM cells infiltrated the brain tissue, implying early glial modifications including an increase in Cx43 expression and distribution. Furthermore, the blockage of Cx43 hemichannels was accompanied by morphological changes and polarization of human GBM cells, typical for migration phenomena. The present study sheds light on the dynamics of GBM cells spreading in the living brain tissue, suggesting that the progression of the tumor correlates with changes within the host brain. Our findings identify the upregulation of Cx43 expression as a highly consistent modification in both mouse and human tissue that may be crucial for GBM infiltration.

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Virtuoso, A., Milior, G., De Luca, C., Moulard, J., Riccio, L., Evstratova, A., Venard, A., Cirillo, G., Pallud, J., Huberfeld, G., Rouach, N., Papa, M.. 2025-12-18. Mapping glioblastoma spreading: connexin43 and glial dynamic in mouse and human glioblastoma microenvironment. https://doi.org/10.64898/2025.12.16.694626

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