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bioRxiv · 10.64898/2025.12.15.694472

Functional Equivalence of Gamma- and X-Ray Irradiation for Long-Term Hematopoietic and AML Transplant Outcomes in Mice

David, S.·Nye, G. J.·Bolcun-Filas, E.·Trowbridge, J.·Young, K. A.
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Abstract

Total-body irradiation (TBI) is routinely used for myeloablation prior to mouse hematopoietic cell transplant. Widespread transition from 137Cs{gamma} -irradiators to X-ray systems has raised questions about whether these modalities yield equivalent biological outcomes. Although prior studies compared{gamma} and X-ray irradiation in healthy syngeneic transplants, their performance in reciprocal congenic models and in primary acute myeloid leukemia (AML) transplant remains unclear. Here, we systematically evaluated{gamma} and X-ray irradiation across dose and dose-rate conditions, and tested dose equivalents in CD45.1/CD45.2 reciprocal transplants and in AML transplant models. While each modality exhibited distinct early effects, both ultimately supported comparable long-term donor chimerism in congenic transplants and equivalent AML engraftment, leukemic burden, and disease progression. These findings indicate that, with proper dose calibration, X-ray irradiation is a functionally effective alternative to{gamma} -irradiation for normal and malignant transplant studies.

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David, S., Nye, G. J., Bolcun-Filas, E., Trowbridge, J., Young, K. A.. 2025-12-17. Functional Equivalence of Gamma- and X-Ray Irradiation for Long-Term Hematopoietic and AML Transplant Outcomes in Mice. https://doi.org/10.64898/2025.12.15.694472

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