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bioRxiv · 10.64898/2025.12.13.694165

Ribosomal Epistatic Interactions Drive the Emergence of High-Level Streptomycin Resistance in Escherichia coli

Xu, F.·Xing, Y.·Men, Y.
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Abstract

The widespread use of antibiotics has raised serious concerns about the emergence and dissemination of antibiotic resistance in the environment. Studies reveal that environmental exposures to antibiotics and other non-antibiotic pollutants can promote the development of high-level antibiotic resistance via de novo mutations. However, specific genotypes conferring strong resistance in resistant mutants, particularly the roles of co-occurring mutations, remained poorly understood. This study fills this knowledge gap by constructing site-specific Escherichia coli mutants and demonstrating that it was the epistatic interactions that conferred the observed strong streptomycin resistance in native E. coli isolates. Three ribosomal mutations (rpsED142N, rpsLR86S and rsmGW150fs), which co-evolved under environmentally relevant selection pressures, acted synergistically to confer resistance far exceeding that of each individual mutation. The interactions between the rsmG frameshift mutation and other reported rpsL point mutations were also investigated. The findings unravel the essential but previously overlooked role played by specific co-evolving mutations in the acquisition of both high-level streptomycin resistance and fitness compensation. The findings also underscore a strong and increasing need to investigate whole genomes of antibiotic-resistant bacteria and identify potential epistatic mutations conferring resistance phenotypes. The integration of those genetic mutations as antibiotic resistance biomarkers will complement with the resistome profiling and enable more accurate antibiotic resistance monitoring and environmental risk assessment. SynopsisMultiple ribosomal mutations co-evolved under environmentally relevant exposure conferred high-level streptomycin resistance via epistatic interactions. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=132 SRC="FIGDIR/small/694165v1_ufig1.gif" ALT="Figure 1"> View larger version (15K): org.highwire.dtl.DTLVardef@e47b2borg.highwire.dtl.DTLVardef@525e27org.highwire.dtl.DTLVardef@1a36e4dorg.highwire.dtl.DTLVardef@9eaf34_HPS_FORMAT_FIGEXP M_FIG Table of Contents Graphic C_FIG

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Xu, F., Xing, Y., Men, Y.. 2025-12-14. Ribosomal Epistatic Interactions Drive the Emergence of High-Level Streptomycin Resistance in Escherichia coli. https://doi.org/10.64898/2025.12.13.694165

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