Dopamine biosynthesis in plants

Ding, Y.; Zhai, Y.; Bruna, T.; Greenblum, S.; Lei, L.; Baumgart, L.; Wang, P.; Novak, V.; Shao, M.; Lee, S. J.; Hazen, S. P.; Kosina, S. M.; Vogel, J.; Northen, T.

doi:10.64898/2025.12.10.693491

bioRxiv · 10.64898/2025.12.10.693491

Dopamine biosynthesis in plants

Ding, Y.·Zhai, Y.·Bruna, T.·Greenblum, S.·Lei, L.·Baumgart, L.·Wang, P.·Novak, V.·Shao, M.·Lee, S. J.·Hazen, S. P.·Kosina, S. M.·Vogel, J.·Northen, T.

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Abstract

Dopamine, an essential human neurotransmitter, also plays important roles in plant-microbe interactions and abiotic stress tolerance. While dopamine biosynthesis is well-characterized in mammals, it remains largely unclear in plants. Through metabolite-based genome-wide association studies, we identified two putative polyphenol oxidase genes for dopamine biosynthesis in Brachypodium distachyon, located in the same chromosomal region as two tyrosine decarboxylase genes. Biochemical and knockout mutant analyses demonstrated that dopamine biosynthesis proceeds primarily via tyramine as an intermediate and contributes to plant tissue browning. Homology searches and functional assays indicate that this dopamine biosynthetic pathway is conserved across diverse plant species. This discovery provides valuable insights into plant dopamine biosynthesis with potential applications in reducing food browning, improving stress resilience, and bioengineering valuable compounds.

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Ding, Y., Zhai, Y., Bruna, T., Greenblum, S., Lei, L., Baumgart, L., Wang, P., Novak, V., Shao, M., Lee, S. J., Hazen, S. P., Kosina, S. M., Vogel, J., Northen, T.. 2025-12-13. Dopamine biosynthesis in plants. https://doi.org/10.64898/2025.12.10.693491

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