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bioRxiv · 10.64898/2025.12.10.692544

Small Molecule Binding to EDS1/PAD4 in LRR-RP-Mediated Pattern-Triggered Immunity

Fliegmann, J.·Janocha, D.·Hua, C.·von Roepenack-Lahaye, E.·Stahl, M.·Locci, F.·Parker, J. E.·Nuernberger, T.·Zhang, L.
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Abstract

Pattern-triggered immunity (PTI) is a key defense mechanism initiated when pathogen-associated molecular patterns are recognized by receptors, including leucine-rich repeat receptor proteins (LRR-RPs)1,2. EDS1 and PAD4 are crucial for LRR-RP-mediated PTI activation3. Small molecule (SM) phosphoribosyl-AMP/ADP, generated by TIR-domain NADase activity, promote the recruitment of helper NLRs into EDS1-PAD4 complex4,5. Here, we show that BAK1 recruitment to LRR-RP complexes is unaffected in eds1-2 or pad4-1 mutants, but is abolished in the sobir1-12 mutant, highlighting the essential role of SOBIR1 in surface immune receptor complex formation. Furthermore, SM-binding to EDS1 and PAD4 is critical for LRR-RP-mediated PTI, as SM-binding-deficient mutants fail to restore immune responses. De novo SM synthesis is required for late PTI responses, including PR1 expression and bacterial resistance, but not for early responses such as ROS and ethylene production. The TNL SADR1 functions only in late PTI responses, suggesting distinct roles of multiple TIR-domain proteins in PTI signaling.

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Fliegmann, J., Janocha, D., Hua, C., von Roepenack-Lahaye, E., Stahl, M., Locci, F., Parker, J. E., Nuernberger, T., Zhang, L.. 2025-12-12. Small Molecule Binding to EDS1/PAD4 in LRR-RP-Mediated Pattern-Triggered Immunity. https://doi.org/10.64898/2025.12.10.692544

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