Early and transient increase in cortical pyramidal cell excitability and delayed alteration of evoked synaptic transmission and t-SNARE proteins content in the hippocampus and neocortex of neonatal and juvenile STXBP1 heterozygous mice

Abstract

De novo mutations in the STXBP1 gene leads to the haploinsufficiency of Munc18.1 protein in patient, and represent one of the major causes of neurodevelopmental disorders including developmental epileptic and non-epileptic encephalopathies. Given the fundamental role of this protein in vesicular exocytosis, most electrophysiological studies have focused on the impact of this haploinsufficiency on synaptic transmission, and much less on intrinsic neuronal properties. Furthermore, the possibility that the electrophysiological consequences may be dependent on the developmental stage has not yet been investigated. Here, we analyze using acute brain slices from neonatal and juvenile STXBP1 heterozygous mice the intrinsic properties as well as spontaneous and evoked glutamatergic and GABAergic synaptic transmission in pyramidal cells located in the CA1 region of the hippocampus and in layers II/III of the motor cortex. We show that Munc18.1 deficiency has different electrophysiological consequences in neonatal (postnatal days, PND 4-7) and juvenile mice (PND30-35). The deficit of Munc18.1 leads to an increase in the intrinsic excitability of hippocampal and motor cortical pyramidal cells in neonates while in juveniles, it is evoked synaptic transmission that is affected, with a greater sensitivity of glutamatergic synapses than of GABAergic synapses in response to high-frequency electrical stimulation. However spontaneous ongoing synaptic activity mediated by glutamate and GABA receptors was unaffected at both stages of development. In addition, we performed western blot analysis and observed that Munc18.1 deficiency in STXBP1 heterozygous mice is associated with decreased t-SNARE proteins expression levels in the hippocampus and neocortex of juvenile but not neonatal mice. Therefore, Munc18.1 deficiency has multiple electrophysiological and biochemical consequences, which depend on the developmental stage. These data suggest also that an alteration in the function of some ion channels is one of the first electrophysiological consequences of the deficit of Munc18.1 and we suggest that the decrease in t-SNARE proteins expression could contribute to the normalization of pyramidal cells firing properties in juvenile.