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bioRxiv · 10.1101/861690

An optimized acetylcholine sensor for monitoring in vivo cholinergic activity

jing, m.·Li, Y.·Zeng, J.·Huang, P.·Skirzewski, M.·Peng, W.·Qian, T.·Tan, K.·Wu, R.·Zhang, S.·Pan, S.·Xu, M.·Li, H.·Saksida, L. M.·Prado, V. F.·Bussey, T.·Prado, M. A. M.·Chen, L.·Cheng, H.
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Abstract

The ability to directly measure acetylcholine (ACh) release is an essential first step towards understanding its physiological function. Here we optimized the GRABACh (GPCR-Activation-Based-ACh) sensor with significantly improved sensitivity and minimal downstream coupling. Using this sensor, we measured in-vivo cholinergic activity in both Drosophila and mice, revealing compartmental ACh signals in fly olfactory center and single-trial ACh dynamics in multiple regions of the mice brain under a variety of different behaviors

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jing, m., Li, Y., Zeng, J., Huang, P., Skirzewski, M., Peng, W., Qian, T., Tan, K., Wu, R., Zhang, S., Pan, S., Xu, M., Li, H., Saksida, L. M., Prado, V. F., Bussey, T., Prado, M. A. M., Chen, L., Cheng, H.. 2019-12-02. An optimized acetylcholine sensor for monitoring in vivo cholinergic activity. https://doi.org/10.1101/861690

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