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bioRxiv · 10.1101/860841

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

Urabe, G.·Shimada, M.·Ogata, T.·Katsuki, S.
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Abstract

Liposomes are widely assumed to present a straightforward physical model of cells. However, almost all previous liposome experiments with pulsed electric fields (PEFs) have been conducted in low-conductivity liquids, a condition that differs significantly from that of cells in medium. Here, we prepared liposomes consisting of soy bean lecithin and cholesterol, at a molar ratio of 1:1, in higher-conductivity liquid that approximated the conditions of red blood cells in phosphate-buffered saline, with inner and outer liquid conductivities of 0.6 and 1.6 S/m, respectively. We found that a single 1.1 kV/cm, 400 s PEF promoted cell-like spontaneous division of liposomes.

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Urabe, G., Shimada, M., Ogata, T., Katsuki, S.. 2019-12-01. Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field. https://doi.org/10.1101/860841

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