Confirmation of local transmission of Orientia tsutsugamushi during scrub typhus outbreaks in Nepal

Dhimal, M.; Dumre, S. P.; Sharma, G. N.; Khanal, P.; Ranabhat, K.; Shah, L. P.; Lal, B. K.; Jha, R.; Upadhyaya, B. P.; Acharya, B.; Shrestha, S. K.; Davidson, S. A.; Charoensinphon, P.; Karki, K. B.

doi:10.1101/852533

bioRxiv · 10.1101/852533

Confirmation of local transmission of Orientia tsutsugamushi during scrub typhus outbreaks in Nepal

Dhimal, M.·Dumre, S. P.·Sharma, G. N.·Khanal, P.·Ranabhat, K.·Shah, L. P.·Lal, B. K.·Jha, R.·Upadhyaya, B. P.·Acharya, B.·Shrestha, S. K.·Davidson, S. A.·Charoensinphon, P.·Karki, K. B.

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Abstract

BackgroundScrub typhus is a severely ignored tropical disease and a leading cause of undifferentiated febrile illness worldwide caused by infection of an obligate intracellular bacteria Orientia tsutsugamushi. It has been rapidly expanding in South Asian countries, although clear epidemiological information is not available from Nepal. After the 2015 earthquake in Nepal, a sudden upsurge in scrub typhus cases was reported. The objective of this study was to investigate scrub typhus and its causative agents in human, rodent and chigger mites to better understand the ongoing transmission ecology. MethodsScrub typhus cases with confirmed diagnosis throughout the country were included in the analysis. Studies were concentrated in the Chitwan district, the site of a major outbreak in 2016. Additional country-wide data from 2015 to 2017 was made available from the government database to analyse the disease distribution using geographical mapping. ResultsDuring 2015-2017, 1,239 scrub typhus cases were confirmed with the largest outbreak occurring in 2016 with 831 (67.1%) cases. The remainder 267 cases were reported in 2017. The case fatality rate was 5.7% in 2015 and declined to 1.1% in 2017. Nationwide outbreak of scrub typhus was identified as the cases were found from 52 of the 75 districts of Nepal. A seasonal trend was observed with a peak during August and September (p = 0.01). In addition to the human cases, the presence of O. tsutsugamushi was also confirmed in rodents and chigger mites from the outbreak areas of southern Nepal. ConclusionThe detection of O. tsutsugamushi in human, rodent, and chigger mites from outbreak locations and wide-spread reports of scrub typhus throughout the country over two years confirms the ongoing transmission of O. tsutsugamushi with a firmly established ecology in Nepal. The countrys health system needs to be strengthened for systematic surveillance, early outbreaks detection, and immediate response actions including treatment and preventive measures. Author SummaryScrub typhus is a disease caused by a bacteria called Orientia tsutsugamushi and transmitted to people through bites of infected chiggers (larval mites). After the 2015 Gorkha earthquake in Nepal, a sudden upsurge in scrub typhus cases was reported with repeated outbreaks from different parts of the country. This study has documted epidemiology of scrub typhus and its causative agents in human, rodent and chigger mites confimring the local transmission O. tsutsugamushi with a firmly established ecology in Nepal. The local transmission of the diseases from most parts of the country demands strengthening for systematic surveillance, early outbreaks detection, and immediate response actions including treatment and preventive measures.

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Dhimal, M., Dumre, S. P., Sharma, G. N., Khanal, P., Ranabhat, K., Shah, L. P., Lal, B. K., Jha, R., Upadhyaya, B. P., Acharya, B., Shrestha, S. K., Davidson, S. A., Charoensinphon, P., Karki, K. B.. 2019-11-22. Confirmation of local transmission of Orientia tsutsugamushi during scrub typhus outbreaks in Nepal. https://doi.org/10.1101/852533

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