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bioRxiv · 10.1101/845768

Nick-seq for single-nucleotide resolution genomic maps of DNA modifications and damage

Cao, B.·Wu, X.·Zhou, J.·Wu, H.·DeMott, M. S.·Gu, C.·Wang, L.·You, D.·Dedon, P. C.
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Abstract

Here we present the Nick-seq platform for quantitative mapping of DNA modifications and damage at single-nucleotide resolution across genomes. Pre-existing breaks are blocked and DNA structures converted to strand-breaks for 3-extension by nick-translation to produce nuclease-resistant oligonucleotides, and 3-capture by terminal transferase tailing. Libraries from both products are subjected to next-generation sequencing. Nick-seq is a generally applicable method illustrated with quantitative profiling of single-strand-breaks, phosphorothioate modifications, and DNA oxidation.

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Cao, B., Wu, X., Zhou, J., Wu, H., DeMott, M. S., Gu, C., Wang, L., You, D., Dedon, P. C.. 2019-11-19. Nick-seq for single-nucleotide resolution genomic maps of DNA modifications and damage. https://doi.org/10.1101/845768

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