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bioRxiv · 10.1101/842484

Transcription closed and open complex formation coordinate expression of genes with a shared promoter region

Hakkinen, A.·Oliveira, S. M. D.·Neeli-Venkata, R.·Ribeiro, A. S.
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Abstract

Many genes are spaced closely, allowing coordination without explicit control through shared regulatory elements and molecular interactions. We study the dynamics of a stochastic model of a gene-pair in a head-to-head configuration, sharing promoter elements, which accounts for the rate-limiting steps in transcription initiation. We find that only in specific regions of the parameter space of the rate-limiting steps is orderly co-expression exhibited, suggesting that successful cooperation between closely spaced genes requires the co-evolution of compatible rate-limiting step configuration. The model predictions are validated by in vivo single-cell, single-RNA measurements of the dynamics of pairs of genes sharing promoter elements. Our results suggest that, in E. coli, the kinetics of the rate-limiting steps in active transcription can play a central role in the dynamics of pairs of genes sharing promoter elements.

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Hakkinen, A., Oliveira, S. M. D., Neeli-Venkata, R., Ribeiro, A. S.. 2019-11-15. Transcription closed and open complex formation coordinate expression of genes with a shared promoter region. https://doi.org/10.1101/842484

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