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bioRxiv · 10.1101/839845

Cell cycle S-phase arrest drives cell extrusion

Dwivedi, V. K.·Pardo-Pastor, C.·Droste, R.·Denning, D. P.·Rosenblatt, J.·Horvitz, H. R.
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Abstract

Cell extrusion is a process of cell elimination in which a cell is squeezed out from its tissue of origin. Extrusion occurs in organisms as diverse as sponges, nematodes, insects, fish and mammals. Defective extrusion is linked to many epithelial disorders, including cancer. Despite broad occurrence, cell-intrinsic triggers of extrusion conserved across phyla are generally unknown. We combined genome-wide genetic screens with live-imaging studies of C. elegans embryos and mammalian epithelial cultures and found that S-phase arrest induced extrusion in both. Cells extruded from C. elegans embryos exhibited S-phase arrest, and RNAi treatments that specifically prevent S-phase entry or arrest blocked cell extrusion. Pharmacological induction of S-phase arrest was sufficient to promote cell extrusion from a canine epithelial monolayer. Thus, we have discovered an evolutionarily conserved cell-cycle-dependent trigger of cell extrusion. We suggest that S-phase-arrest induced cell extrusion plays a key role in physiology and disease.

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Dwivedi, V. K., Pardo-Pastor, C., Droste, R., Denning, D. P., Rosenblatt, J., Horvitz, H. R.. 2019-11-13. Cell cycle S-phase arrest drives cell extrusion. https://doi.org/10.1101/839845

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